Abstract:
Advances in spatial transcriptomics have revolutionized our understanding of cell types and tissue organization, but challenges in resolution, sensitivity, speed, and accessibility limit broader adoption. To overcome these hurdles, we introduce two complementary innovations: SPRINTseq (Spatially Resolved and Signal-Diluted Next-Generation Targeted Sequencing) and PRISM (Profiling of RNA In Situ through Single-round of iMaging). SPRINTseq combines hybrid block coding and molecular dilution to recover over 142 million transcripts from 453,843 cells in less than two days, uncovering subcellular molecular architectures in Alzheimer's disease. PRISM leverages spectral intensity and radius vector coding to enable 64-plex RNA imaging in a single shot using conventional microscopes, achieving subcellular resolution across diverse tissues, including mouse brains, embryos, and human tumors. By tracking embryonic development and revealing the roles of cancer-associated fibroblasts in tumor microenvironments, PRISM extends to quasi-3D and 3D spatial transcriptomics, offering fast, robust, and accessible solutions for high-resolution RNA imaging. Together, these methods push the boundaries of spatial transcriptomics, enabling transformative insights into complex biological processes and diseases.

Bio:
Prof. Yanyi Huang is Professor of Analytical Chemistry at Peking University. He is the Principal Investigator in the Biomedical Pioneering Innovation Center (BIOPIC), the Peking-Tsinghua Center for Life Sciences at Peking University, and the Institute of Chemical Biology at Shenzhen Bay Laboratory. He received his BS and ScD degrees from Peking University, conducted postdoc research at Caltech and Stanford University, and started his independent career in 2006 at Peking University. He is Fellow of the Royal Society of Chemistry. He is working on technology development for integrative biology research, especially the new methods for genomic sequencing and large-scale microfluidics.