Abstract:
The current progresses of single-cell sequencing and spatiotemporal transcriptome enable scientists not only can discover the specific cell types in tissue, but also can detect the cell distribution in the spatial dimension. Taking advantages of a newly developed spatial-temporal transcriptomics approach—SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq) by BGI-research, with the highest profiling resolution to data, we studied the cellular and molecular dynamics during organ regeneration process in Axolotl, an animal that can regenerate damaged appendages and multiple internal organs, including the brain. Employing Stereo-seq, we generated a group of spatial transcriptomic data of telencephalon sections that covered six developmental and seven injury-induced regenerative stages. We discovered a sub-population of progenitor cells may then proliferate to cover the wound area and subsequently replenish lost neurons. Interestingly, we also observed that regeneration of the axolotl telencephalon exhibited similar neurogenesis patterns to development, suggesting that brain regeneration partially recapitulates the development process. Our spatial transcriptomic data highlights the cellular and molecular features of the axolotl telecephalon during development and injury-induced regeneration. 

Details 
- Introduction of BGI’s single-cell and spatial omics (Stereo-seq) technology. 
- The application of Stereo-seq in the study of axolotl brain regeneration (Wei et al.,Science, 2022).
- Current progresses in the development of Stereo-seq V2 and spatial multi-oimcs technology.

Bio:
Ying Gu, Ph.D.,Principal Investigator and Deputy Director at BGI. Her expertise lies in cell and molecular biology, developmental biology, stem cell research, and gene editing technologies, with a particular focus on single-cell genomics. Dr. Gu employs cutting-edge methodologies such as single-cell and spatiotemporal omics to understand the early embryonic development across different species, explore the competitive dynamics within species chimerism, and elucidate the molecular and cellular underpinnings of limb and brain regeneration in salamanders. Her research has been featured in top-tier academic journals, including Science, Cell, Nature, Cell Stem Cell, Cell Discovery, Developmental Cell, and Protein & Cell.